Human CellExp™ IL-2 R beta /CD122, human recombinant
- Usually Shipped in 5 Working Days
- 10 μg
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Biomolecule/Target: IL-2 R beta /CD122
Synonyms: IL2RB, RP5-1170K4.6, CD122, P70-75
Alternates names: IL2RB, RP5-1170K4.6, CD122, P70-75
Taglines: Involved in receptor mediated endocytosis
Country of Animal Origin: USA
NCBI Gene ID #.: 3560
NCBI Gene Symbol: IL2RB
Gene Source: Human
Accession #: P14784
Source: HEK293 cells
Purity by SDS-PAGE #: ≥90%
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5 - 2.5 µg/mL in the presence of 4.0 ng/mL of recombinant human IL-15.
Results: Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.5 - 2.5 µg/mL in the presence of 4.0 ng/mL of recombinant human IL-15.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.4 kDa. The predicted N-terminus is Ala 27. DTT-reduced Protein migrates as 40 kDa due to glycosylation.
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. CD122 also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans