Human CellExp™ IL-6 Rα, Human Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Biomolecule/Target: IL-6 Rα
Synonyms: soluble IL-6 receptor alpha, B cell stimulatory factor-2, CD126
Alternates names: soluble IL-6 receptor alpha, B cell stimulatory factor-2, CD126
Taglines: A type I cytokine receptor
Country of Animal Origin: USA
NCBI Gene ID #.: 3570
NCBI Gene Symbol: IL6RA
Gene Source: Human
Accession #: P08887
Source: HEK293 cells
Purity by SDS-PAGE #: ≥98%
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method): Determined by its ability to intensify the IL-6 induced growth inhibition of mouse M1 cells. The expected ED50 is ≤ 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.
Biological activity: Determined by its ability to intensify the IL-6 induced growth inhibition of mouse M1 cells. The expected ED50 is ≤ 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: ~37.6 kDa
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS pH 7.2.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Background Information: IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. The IL-6Rα chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6Rα. The gp130 also can transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6Rα which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6Rα is a 37.6 kDa protein consisting of the extracellular domain of the IL-6Rα chain (339 amino acid residues).
Amino acid sequence: LAPRRCPAQE VARGVLTSLP GDSVTLTCPG VEPEDNATVH WVLRKPAAGS HPSRWAGMGR RLLLRSVQLH DSGNYSCYRA GRPAGTVHLL VDVPPEEPQL SCFRKSPLSN VVCEWGPRST PSLTTKAVLL VRKFQNSPAE DFQEPCQYSQ ESQKFSCQLA VPEGDSSFYI VSMCVASSVG SKFSKTQTFQ GCGILQPDPP ANITVTAVAR NPRWLSVTWQ DPHSWNSSFY RLRFELRYRA ERSKTFTTWM VKDLQHHCVI HDAWSGLRHV VQLRAQEEFG QGEWSEWSPE AMGTPWTESR SPPAENEVST PMQALTTNKD DDNILFRDSA NATSLPVQD
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans