Human CellExp™ IL-4 Rα, Human Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Biomolecule/Target: IL-4 Rα
Synonyms: soluble IL-4 receptor alpha, CD124
Alternates names: soluble IL-4 receptor alpha, CD124
Taglines: A receptor for both interleukin 4 and interleukin 13
Country of Animal Origin: USA
NCBI Gene ID #.: 3566
NCBI Gene Symbol: IL4RA
Gene Source: Human
Accession #: P24394
Source: HEK293 cells
Purity by SDS-PAGE #: ≥98%
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method): Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.
Biological activity: Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: ~24 kDa
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS pH 7.0.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to - 80°C
Background Information: IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain (γc). The type I receptor contains in addition to the γc an IL-4Rα subunit, whereas the type II receptor contains the IL-13Rα. The secreted extracellular domain of IL-4Rα, called sIL-4Rα, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naïve CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4Rα is a 209 amino acid protein which corresponds to the entire extracellular domain of IL- 4Rα.
Amino acid sequence: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans