Human CellExp™ Kallikrein-13, human recombinant

Biomolecule/Target: Kallikrein-13

Synonyms: KLK13, KLKL4, KLK-13, Kallikrein-13

Alternates names: KLK13, KLKL4, KLK-13, Kallikrein-13

Taglines: Regarded as a novel cancer biomarker

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 26085

NCBI Gene Symbol: KLK13

Gene Source: Human

Accession #: Q9UKR3

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is > 150 pmoles / min / µg.

Results: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is > 150 pmoles / min / µg.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 28 kDa. The predicted N-terminus is Gly 18. DTT-reduced Protein migrates as 33-40 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, pH 6.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Kallikrein-13 (KLK13) is also known as Kallikrein-like protein 4 (KLK-L4), is a secreted protein, belongs to the peptidase S1 family and Kallikrein subfamily. KLK-13 contains one peptidase S1 domain. KLK13 is mainly expressed in prostate, breast, testis and salivary gland. KLK13 may be involved in the pathogenesis and / or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans