Human CellExp™ R spondin-1, human recombinant
- Usually Shipped in 5 Working Days
- 10 μg
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Biomolecule/Target: R spondin-1
Synonyms: RSPO1, RSPO-1, CRISTIN3, CRISTIN-3, FLJ40906, RP11-566C13.1, RSPO, R-spondin-1, Rspondin-1, Rspondin1, Rspondin.
Alternates names: RSPO1, RSPO-1, CRISTIN3, CRISTIN-3, FLJ40906, RP11-566C13.1, RSPO, R-spondin-1, Rspondin-1, Rspondin1, Rspondin.
Taglines: Regulates Wnt/β-catenin signaling
Country of Animal Origin: USA
NCBI Gene ID #.: 284654
NCBI Gene Symbol: RSPO1
Gene Source: Human
Accession #: Q2MKA7
Source: HEK293 cells
Purity by SDS-PAGE #: ≥98%
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml ( 100 μl/well ) can bind human LIMPII with a linear ranger of 31.25 - 1000 ng/ml.
Results: Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml ( 100 μl/well ) can bind human LIMPII with a linear ranger of 31.25 - 1000 ng/ml.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with a 6×his tag at C-terminal, and has a calculated molecular mass of 27.6 kDa. The predicted N-terminal is Ser 21. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhRSPO1 is approximately 40 kDa due to the glycosylation.
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: R-spondin-1, also known as Roof plate-specific Spondin 1 (RSPO1) and cysteine rich and single thrombospondin domain containing protein 3 (Cristin 3), is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. All R spondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteine rich furin like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin like domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively. R-Spondin-1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY. R-Spondin-1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK1 for binding to the Wnt co receptors, Kremen and LRP6, reducing their DKK1 mediated internalization. Reports differ on whether R-spondin 1 binds LRP6 directly.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans