Human CellExp™ VCAM-1, Human Recombinant

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SKU:
26-7211
Availability:
Usually Shipped in 5 Working Days
Storage Temperature:
-20°C
Shipping Conditions:
Gel Pack
Shelf Life:
12 months
€154.00 - €425.00
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Description

Biomolecule/Target: VCAM1

Synonyms: CD106, INCAM-100, MGC108734, MGC99561, VCAM, VCAM1, VCAM1B, VECAM1

Alternates names: CD106, INCAM-100, MGC108734, MGC99561, VCAM, VCAM1, VCAM1B, VECAM1

Taglines: Mediates leukocyte-endothelial cell adhesion and signal transduction

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 7412

NCBI Gene Symbol: VCAM1

Gene Source: Human

Accession #: P19320

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.

Biological activity: Determined by its ability to support the adhesion of human U937 cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~74.0 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Background Information: VCAM is a 110 kDa cell surface integral membrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. The primary function of VCAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 may play a vital role in the development several diseases, including atherosclerosis and rheumatoid arthritis. The human VCAM-1 gene codes for a 715 amino acid transmembrane glycoprotein containing a 19 amino acid cytoplasmic domain, a 22 amino acid transmembrane domain, and a 674 amino acid extracellular domain. Recombinant human VCAM-1 is a 74.0 kDa glycoprotein comprising the extracellular domain (673 amino acid residues) of VCAM-1. Monomeric glycosylated VCAM-1 migrates at an apparent molecular weight of approximately 90.0 kDa by SDS-PAGE analysis.

Amino acid sequence: FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYS FPKDPEIHLS GPLEAGKPIT VKCSVADVYP FDRLEIDLLK GDHLMKSQEF LEDADRKSLE TKSLEVTFTP VIEDIGKVLV CRAKLHIDEM DSVPTVRQAV KELQVYISPK NTVISVNPST KLQEGGSVTM TCSSEGLPAP EIFWSKKLDN GNLQHLSGNA TLTLIAMRME DSGIYVCEGV NLIGKNRKEV ELIVQEKPFT VEISPGPRIA AQIGDSVMLT CSVMGCESPS FSWRTQIDSP LSGKVRSEGT NSTLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFP RDPEIEMSGG LVNGSSVTVS CKVPSVYPLD RLEIELLKGE TILENIEFLE DTDMKSLENK SLEMTFIPTI EDTGKALVCQ AKLHIDDMEF EPKQRQSTQT LYVNVAPRDT TVLVSPSSIL EEGSSVNMTC LSQGFPAPKI LWSRQLPNGE LQPLSENATL TLISTKMEDS GVYLCEGINQ AGRSRKEVEL IIQVTPKDIK LTAFPSESVK EGDTVIISCT CGNVPETWII LKKKAETGDT VLKSIDGAYT IRKAQLKDAG VYECESKNKV GSQLRSLTLD VQGRENNKDY FSP

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans

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