Human CellExp™ Recombinant Ebolavirus BDBV Envelope Glycoprotein 1 (GP1)
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: GP1, GP, Envelope glycoprotein, GP2 (subtype Bundibugyo,strain Uganda 2007)
Alternates names: GP1, GP, Envelope glycoprotein, GP2
Country of Animal Origin: USA
NCBI Gene ID #.: 9487265
NCBI Gene Symbol: GP
Gene Source: Bundibugyo virus
Accession #: B8XCN0
Source: HEK 293 cells
Purity by SDS-PAGE #: > 95%
Assay #2: N/A
Endotoxin Level: < 1.0 EU per/μg
Activity (Specifications/test method): N/A
Biological activity: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 31.8 kDa
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
Reconstitution Instructions: Reconstitute in 1X PBS to the desired protein concentration.
Background Information: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans