Human CellExp™ TIGIT, Fc Tag, Mouse Recombinant

Biomolecule/Target: Tigit

Synonyms: TIGIT, VSIG9, VSTM3

Alternates names: TIGIT, VSIG9, VSTM3

Taglines: >95% Pure, Human cell expressed TIGIT

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 100043314

NCBI Gene Symbol: Tigit

Gene Source: Mouse

Accession #: A0A0B4J1G6

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/μg

Activity (Specifications/test method): Measured by its ability to converts 1 μmole of L-methionine and ATP to S-adenosyl-L-methionine (AdoMet), inorganic phosphate (Pi), and pyrophosphate (PPi) at 37ºC and pH 7

Biological activity: Immobilized Mouse TIGIT, Fc Tag at 5μg/mL (100 μL/well) can bind Human Nectin-2, His Tag with a linear range of 2.5-20 μg/mL.

Results: N/A

Binding Capacity: Immobilized Mouse TIGIT, Fc Tag at 5μg/mL (100 μL/well) can bind Human Nectin-2, His Tag with a linear range of 2.5-20 μg/mL.

Unit Definition: N/A

Molecular Weight: 40 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3), which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans