Human CellExp™ TIGIT, human recombinant

Biomolecule/Target: TIGIT

Synonyms: TIGIT, VSIG9, VSTM3

Alternates names: TIGIT, VSIG9, VSTM3

Taglines: >95% Pure, Human cell expressed TIGIT

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 201633

NCBI Gene Symbol: TIGIT

Gene Source: Human

Accession #: Q495A1

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per μg of the protein by the LAL method.

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 15 kDa. The predicted N-terminus is Met 22. DTT-reduced Protein migrates as 18-24 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Amino acid sequence: Met 22 – Pro 141

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans