Biovision
Human CellExp™ Tim-3, (Fc-Tag Mouse), Mouse Recombinant
- SKU:
- 26-P1360
- Availability:
- Usually Shipped in 5 Working Days
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: Tim-3
Synonyms: TIM3; TIMD3; Hepatitis A Virus Cellular Receptor 2; HAVcr-2; T Cell Immunoglobulin and Mucin Domain-containing Protein 3, HAVCR2
Alternates names: TIM3; TIMD3; Hepatitis A Virus Cellular Receptor 2; HAVcr-2; T Cell Immunoglobulin and Mucin Domain-containing Protein 3, HAVCR2
Taglines: Mediate phagocytosis of apoptotic cells and inhibit aggressive Th1-mediated auto- and alloimmune responses.
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 171285
NCBI Gene Symbol: HAVCR2
Gene Source: Mouse
Accession #: Q3KP82
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGE #: ≥98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <5 EU/μg by LAL method
Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.
Biological activity: Immobilized recombinant human Galectin-9 at 500 ng/ml can bind Tim-3 mouse recombinant with an apparent KD <10nM.
Results: N/A
Binding Capacity: Immobilized recombinant human Galectin-9 at 500 ng/ml can bind Tim-3 mouse recombinant with an apparent KD <10nM.
Unit Definition: N/A
Molecular Weight: 65kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.2 μm-filtered solution in PBS
Reconstitution Instructions: Reconstitute with 100 µl sterile water. Add 1X PBS to the desired protein concentration.
Background Information: The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is preferentially expressed on Th1 and Tc1 cells, and generates an inhibitory signal resulting in apoptosis of Th1 and Tc1 cells. TIM-3 is also expressed on some dendritic cells and can mediate phagocytosis of apoptotic cells and cross-presentation of antigen. Tim-3 functions to inhibit aggressive Th1-mediated auto- and alloimmune responses. Tim-3 pathway blockade by administration of Tim-3:Fc fusion protein accelerates diabetes in nonobese diabetic mice, causes hyperproliferation of Th1 cells and Th1 cytokine release in an experimental autoimmune encephalomyelitis (EAE) model and prevents acquisition of transplantation tolerance induced by costimulation blockade
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans