Human CellExp™ TIM-3 / HAVCR2, Cynomolgus Recombinant

Biomolecule/Target: N/A

Synonyms: HAVCR2, TIM3, TIMD3, FLJ14428, KIM3

Alternates names: HAVCR2, TIM3, TIMD3, FLJ14428, KIM3

Taglines: Mediate phagocytosis of apoptotic cells and inhibit aggressive Th1-mediated auto- and alloimmune responses.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: N/A

NCBI Gene Symbol: N/A

Gene Source: Cynomolgus

Accession #: EHH54703

Recombinant: FALSE

Source: HEK 293 cells

Purity by SDS-PAGE #: >90% as determined by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method

Activity (Specifications/test method): Immobilized Cynomolgus TIM-3 at 1 μg/mL (100 μL/well) can bind Anti-TIM-3 Mab with a linear range of 0.8-6 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: Immobilized Cynomolgus TIM-3 at 1 μg/mL (100 μL/well) can bind Anti-TIM-3 Mab with a linear range of 0.8-6 ng/mL

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.6 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water. Do not vortex. It is recommended to store at -20°C.

Background Information: Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Amino acid sequence: AA Ser 22 - Arg 201

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans