Human CellExp™ KIM3/HAVCR2, human recombinant

Biomolecule/Target: KIM3/HAVCR2

Synonyms: HAVCR2, TIM3, TIMD3, FLJ14428, KIM3

Alternates names: HAVCR2, TIM3, TIMD3, FLJ14428, KIM3

Taglines: A Th1-specific cell surface protein that regulates macrophage activation.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 84868

NCBI Gene Symbol: HAVCR2

Gene Source: Human

Accession #: Q8TDQ0

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD<30 nM.

Results: Measured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD<30 nM.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with the Fc region of human IgG1 at the C-terminus and has a calculated MW of 42 kDa after removal of signal peptide. In DTT-reduced SDS-PAGE, rhKIM3-Fc protein migrates as 45 kDa poly peptide due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Hepatitis A virus cellular receptor 2 also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans