Biovision
Human CellExp™ TLR-3, Human Recombinant
- SKU:
- 26-7220
- Availability:
- Usually Shipped in 5 Working Days
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: TLR3
Synonyms: Toll-like receptor 3; CD283 antigen
Alternates names: Toll-like receptor 3; CD283 antigen
Taglines: Promotes the NF-kB pathway to initiate innate and adaptive immune responses
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 7098
NCBI Gene Symbol: TLR3
Gene Source: Human
Accession #: O15455
Recombinant: Yes
Source: HEK 293 cells
Purity by SDS-PAGE #: ≥95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method): Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50 for this effect is ≤ 30 μg/ml in the presence of 100 μg/ml of Poly I:C.
Biological activity: Determined by its ability to inhibit IL-8 secretion induced by Poly I:C in the TLR3 receptor-expressing HEK293 cells (HEK293 TLR3r). The ED50 for this effect is ≤ 30 μg/ml in the presence of 100 μg/ml of Poly I:C.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 77.4 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate, pH 7.5.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Background Information: TLR-3 is a single-pass type I receptor that binds to and signals the presence of microbial pathogens and double stranded RNA (dsRNA) viruses. Signaling through TLR-3 can promote the NF-¬B pathway to initiate innate and adaptive immune responses to bacterial and viral infections, as well as the p53 pathway to trigger apoptosis in cells infected with dsRNA viruses. TLR-3 belongs to a family of structurally-related toll-like receptors (TLRs) containing an N-terminal domain rich in leucine repeats and a C-terminal intracellular Toll/interleukin (IL)-1 (TIL) domain. TLR-3 is expressed primarily in dendritic cells of the placenta and pancreas where it can reside on both sides of the plasma membrane and in the endosomal compartment of the cells. Recombinant human TLR-3 is 77.4 kDa glycoprotein containing 681 residues which comprise the TLR-3 extracellular domain.
Amino acid sequence: STTKCTVSHE VADCSHLKLT QVPDDLPTNI TVLNLTHNQL RRLPAANFTR YSQLTSLDVG FNTISKLEPE LCQKLPMLKV LNLQHNELSQ LSDKTFAFCT NLTELHLMSN SIQKIKNNPF VKQKNLITLD LSHNGLSSTK LGTQVQLENL QELLLSNNKI QALKSEELDI FANSSLKKLE LSSNQIKEFS PGCFHAIGRL FGLFLNNVQL GPSLTEKLCL ELANTSIRNL SLSNSQLSTT SNTTFLGLKW TNLTMLDLSY NNLNVVGNDS FAWLPQLEYF FLEYNNIQHL FSHSLHGLFN VRYLNLKRSF TKQSISLASL PKIDDFSFQW LKCLEHLNME DNDIPGIKSN MFTGLINLKY LSLSNSFTSL RTLTNETFVS LAHSPLHILN LTKNKISKIE SDAFSWLGHL EVLDLGLNEI GQELTGQEWR GLENIFEIYL SYNKYLQLTR NSFALVPSLQ RLMLRRVALK NVDSSPSPFQ PLRNLTILDL SNNNIANIND DMLEGLEKLE ILDLQHNNLA RLWKHANPGG PIYFLKGLSH LHILNLESNG FDEIPVEVFK DLFELKIIDL GLNNLNTLPA SVFNNQVSLK SLNLQKNLIT SVEKKVFGPA FRNLTELDMR FNPFDCTCES IAWFVNWINE THTNIPELSS HYLCNTPPHY HGFPVRLFDT SSCKDSAPFE L
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans