CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit

Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.

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Tag Line: Measures Activated Caspases in intact cells by FACS

Summary: • Detection method- Flow cytometry, L-1 channel (Ex/Em = 488/530 nm). • Species reactivity- Mammalian • Kit size- Convenient sizes (25 and 100 assays) • Applications- Detection of activated caspases in living cells.

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Sample Type: Live cells

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Features & Benefits: • Simple one-step procedure; takes only 30 minutes • Fast and convenient • D₂R is more cell-permeable than other fluorometric caspase substrates