EZDetect™ Aldo-keto Reductase Activity Assay Kit (Colorimetric)

Aldo-keto Reductases (AKRs) are a superfamily of NAD(P)H linked oxidoreductases which contains more than 190 members. A complete listing of AKR superfamily can be found at: https://www.med.upenn.edu/akr/. The common function of AKR superfamily is to oxidize/reduce aldehydes or ketones to their corresponding primary or secondary alcohols. Due to their broad substrate specificity, AKRs play roles in intermediary metabolism, biosynthesis and detoxification. In humans, the AKRs are not only involved in carbonyl metabolism, but they also regulate signaling through nuclear receptors which leads to chemical carcinogenesis. Many studies demonstrate increasing expression of AKRs in cancers such as lung, liver and colon cancers. BioVision’s EZDetect™ Aldo-keto Reductase Activity Assay Kit provides a convenient tool for sensitive detection of AKR activity in a variety of samples. In this assay, AKR acts on a general substrate and converts NADP+ to NADPH. This NADPH reacts with the AKR probe and generates color. This color is proportional to the activity of AKR in the sample. This kit can detect AKR activity as low as 0.001 mU in samples and has been validated with AKR1B10, AKR1C1 and AKR1C3. Further tests are in progress for other members of the AKR family.

Alternate Name: AKR activity assay kit

Tag Line: Market's first enzymatic AKR activity assay. Simple and Ultrasensitive.

Summary: Simple and Ultrasensitive assay to colorimetrically measure as low as 0.001 mU of AKR activity.

Detection Method: Absorbance (450 nm)

Sample Type: • Animal tissues: such as liver, pancreas, small intestine, etc. • Cells: Adherent and suspension cells. • Biological fluids: such as serum and plasma.

Species Reactivity: Mammalian

Applications: • Measurement of aldo-keto reductase activity in various tissues/cells. • Screening of aldo-keto reductase inhibitors.

Features & Benefits: Simple, ultra-sensitive assay. Colorimetric detection