EZScreen™ Beta-Lactamase Activity Colorimetric Assay Kit (384-well)

Beta-Lactamases (βLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. βLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as β-lactams. Antibiotics containing β-lactam rings (i.e. penicillin, cephalosporin, monobactam, carbapenem) are highly susceptible to be hydrolyzed via enzymatic activity, which deactivates their antibiotic potency. βLs have become a significant clinical threat due to the alarming number of cases of bacterial strains showing β-lactam antibiotic resistance. Approaches for combatting this type of resistance have opened up the possibility of developing a new form of β lactam antibiotics that are more resistant to β-Lactamase enzymatic activity. Therefore, experimental research has been arising in wide extent for developing new drugs or inhibitors effective against the β-lactam antibiotic resistance bacteria. BioVision’s EZScreenTM Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD: 490 nm), which is directly proportional to the amount of βL activity. The assay can detect enzymatic activity as low as 15 mU in a variety of biological samples

Alternate Name: N/A

Tag Line: Highly Sensitive, no sample preparation required

Summary: • Detection method: Absorbance (490 nm) • Application: - Measurement of β-Lactamase activity in various biological samples - Analysis of β-Lactamase activity in pathological conditions

Detection Method: Absorbance (490 nm)

Sample Type: • Serum, urine, saliva from mammals infected with βL-secreting bacteria • Food (e.g. milk) • Fermentation media, bacterial cultures, etc.

Species Reactivity: N/A

Applications: • Measurement of β-Lactamase activity in various biological samples • Analysis of β-Lactamase activity in pathological conditions

Features & Benefits: • Rapid, simple & sensitive • The assay can detect enzymatic activity as low as 15 mU in a variety of biological samples