EZScreen™ Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well)

Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction: α-ketoglutarate + alanine ⇌ glutamate + pyruvate It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well.

Alternate Name: N/A

Tag Line: Sensitive Assay, HTS

Summary: • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Mammalian • Applications- The kit detects ALT with a detection limit of 0.1 µU per well.

Detection Method: Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)

Sample Type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food, etc.

Species Reactivity: Eukaryotes

Applications: • Evaluation of pentose phosphate pathway

Features & Benefits: • Simple procedure • Fast and convenient • Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.