QuickDetect™ CD40 Ligand (Human) ELISA Kit

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. BioVision’s QuickDetect™ CD40 Ligand ELISA kit is a sandwich ELISA assay for measuring the quantity of human CD40 Ligand in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash steps respectively, resulting in less errors and more consistent results.

Alternate Name: N/A

Tag Line: A rapid sandwich ELISA kit for the quantitative measurement of CD40 Ligand in human serum, plasma, cell culture supernatnat and other biological fluids

Summary: • Detection method- Absorbance (450 nm)• Species reactivity- Human• Application- quantitative measurement of CD40L in human serum, plasma, cell culture supernatant, and other biological fluid.

Detection Method: Absorbance (450 nm)

Sample Type: Human serum, plasma, tissue homogenates and other biological fluids.

Species Reactivity: Human

Applications: This ELISA kit is used for quantitative measurement of CD40L in human serum, plasma, cell culture supernatant and other biological fluids.

Features & Benefits: Easy, convenient and time-saving method to measure the level of CD40 Ligand in human serum, plasma, culture supernatants and other biological fluids. The entire assay can be performed within 90 minutes with with fewer incubation and wash step respectively, resulting in less errors and more consistent results. Detection Range: 45 - 4000 pg/ml Sensitivity: < 14 pg/ml Assay Precision: Intra-Assay: CV < 10%; Inter-Assay: CV < 12% (CV (%) = SD/mean X 100) Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.