ToxOut™ Endotoxin-Free Protein A Sepharose

Endo toxin free Protein A-Sepharose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Sepharose beads under proprietary endotoxin-free conditions. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Sepharose is ≥ 15 mg human or rabbit IgG per ml of wet beads. Protein A-Sepharose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation. Our Endotoxin-free Protein A-Sepharose also shows ability of reducing/removing certain amount of endotoxin from serum or ascites samples.

Sort Name: Protein A Sepharose (Endotoxin-free)

Taglines: Reusable, Endotoxin free Protein A conjugated Sepharose beads with ≥15 mg/ml binding capacity & 0.85 cm/min flow rate for antibody purification from multiple sources

Product Highlights: • CONTENTS- Supplied as a 50% slurry in 20 % Ethanol/H2O -ENDOTOXIN FREE - HIGH BINDING CAPACITY: Binding of IgG ≥15 mg human or rabbit IgG/ml Protein A-Sepharose. - MINIMAL LEACHING OF THE LIGAND. - FLOW RATE TESTED*: 0.85 cm/min. *Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm). - USAGE: Reusable for up to 10 times without significant loss of binding capacity. • APPLICATIONS - Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.

Label Name: Protein A Sepharose (Endotoxin-free)

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