EZSolution™ DAF-2 diacetate

DAF-2 diacetate is a cell-permeable derivative of DAF-2 (Cat. No. 2752) and a very useful indicator of nitric oxide (NO). Upon entry into the cell, DAF-2 diacetate is converted into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.

Alternate Name/Synonyms: 4,5-Diaminofluorescein diacetate; 2-​(3,​6-​diacetyloxy-​4,​5-​diamino-​9H-​xanthen-​9-​yl)-​benzoic acid; DAF-2 DA

Appearance: Liquid

Appearance: In DMSO (5 mM)

Appearance: 205391-02-2

Appearance: Y

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₄H₁₈N₂O₇

Molecular Weight: 446.41

Cell-Permeable?: Yes

Purity: ≥98% by HPLC

Solubilities: N/A

Handling: Protect from air and light

Country of Origin: USA

Tag Line: A nitric oxide (NO) probe

MDL Number: MFCD05865101

PubChem CID: 6603693

SMILES: CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC(=C(C=C5C(=O)O3)N)N

InChi: InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

InChi Key: PTSUYDXEEKDBQU-UHFFFAOYSA-N

Category: Biochemicals