EZSolution™ DAF-2

DAF-2 is a highly sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.

Alternate Name/Synonyms: 4,5-Diaminofluorescein; 2-​(3,​6-​dihydroxy-​4,​5-​diamino-​9H-​xanthen-​9-​yl)-​benzoic acid

Appearance: Liquid

Appearance: In DMSO (5 mM)

Appearance: 205391-01-1

Appearance: Y

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₀H₁₄N₂O₅

Molecular Weight: 362.34

Cell-Permeable?: No

Purity: ≥95% by HPLC

Solubilities: N/A

Handling: Protect from air and light

Country of Origin: USA

Tag Line: A nitric oxide (NO) probe

MDL Number: MFCD01866182

PubChem CID: 10666340

SMILES: C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O

InChi: InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2

InChi Key: LTYUPYUWXRTNFQ-UHFFFAOYSA-N

Category: Biochemicals