Human CellExp™ ApoJ/Clusterin, human recombinant

Biomolecule/Target: ApoJ/Clusterin

Synonyms: TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2, AAG4, KUB1, SGP2, SP-40, TRPM2, MGC24903.

Alternates names: TRPM-2, Apolipoprotein J, APO-J, CLI, CLU, SGP-2, AAG4, KUB1, SGP2, SP-40, TRPM2, MGC24903.

Taglines: A heavily glycosylated protein upregulated in multiple pathological conditions

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 1191

NCBI Gene Symbol: CLU

Gene Source: Human

Accession #: P10909

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to induce clustering of human clear cell carcinoma epithelial cells Caki-2. Measured by its ability to inhibit DTT (Dithiothreitol) induced BSA precipitation.

Results: Measured by its ability to induce clustering of human clear cell carcinoma epithelial cells Caki-2. Measured by its ability to inhibit DTT (Dithiothreitol) induced BSA precipitation.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused is fused with 6 ×his tag at the C terminus, and has a calculated MW of 50.9 kDa (Asp23-Glu449, α chain 24.5kDa + β chain 27kDa). The recombinant rh CLUS / Clusterin (Asp23-Glu 449) was cleaved into α chain and β chain, which form a heterodimer linked by disulfide bonds. DTT reduced protein migrates as 34-37kDa bands in SDS-PAGE due to glycosylation, corresponding to the cleaved β chain, and α chain respectively.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Native Apolipoprotein J (ApoJ), also named Clusterin, is a heavily glycosylated, 75-80 kDa disulfide-linked heterodimeric protein. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, and membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents. Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans