Human CellExp™ APRIL / TNFSF13, Human Recombinant

Biomolecule/Target: TNFSF13

Synonyms: APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2

Alternates names: APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2

Taglines: Plays a role in the regulation of tumor cell growth.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 8741

NCBI Gene Symbol: TNFSF13

Gene Source: Human

Accession #: O75888

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: Immobilized Human BCMA, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human APRIL, Fc Tag with a linear range of 0.6-10 ng/mL

Results: N/A

Binding Capacity: Immobilized Human BCMA, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human APRIL, Fc Tag with a linear range of 0.6-10 ng/mL

Unit Definition: N/A

Molecular Weight: 42.8 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 100 µg/ml.

Background Information: APRIL (a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans