Human CellExp™ BTLA, Human recombinant

Biomolecule/Target: CD272

Synonyms: BTLA, CD272

Alternates names: BTLA, CD272

Taglines: B- and T-lymphocyte attenuator

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 151888

NCBI Gene Symbol: BTLA

Gene Source: Human

Accession #: NP_001078826.1

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: ≥92%

Assay #2: N/A

Endotoxin Level: < 1 EU/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: Immobilized Human HVEM, C-Twin Strep Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 μg/mL.

Results: N/A

Binding Capacity: Immobilized Human HVEM, C-Twin Strep Tag at 2 ng/ml (100 µl/well),can bind Human BTLA, Fc Tag with a linear of 0.1-0.5 μg/mL.

Unit Definition: N/A

Molecular Weight: This protein is fused with a human IgG1 Fc tag at C-terminus and has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Background Information: B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans