Human CellExp™ CD117 / c-kit, Human Recombinant

Biomolecule/Target: N/A

Synonyms: CD117, SCFR, c-Kit, KIT

Alternates names: CD117, SCFR, c-Kit, KIT

Taglines: Tyrosine-protein kinase that acts as cell-surface receptor

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3815

NCBI Gene Symbol: KIT

Gene Source: Human

Accession #: P10721

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Human CD117, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human SCF, Avitag,His Tag with a linear range of 0.5-8 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.1 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.

Amino acid sequence: AA Gln 26 - Thr 516

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans