Human CellExp™ VEGF-C, human recombinant

Biomolecule/Target: VEGF-C

Synonyms: VEGFC, Flt4-L, VRP

Alternates names: VEGFC, Flt4-L, VRP

Taglines: Is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 7424

NCBI Gene Symbol: VEGF-C

Gene Source: Human

Accession #: P49767

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is typically 0.15-0.7 μg/ml.

Biological activity: Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is typically 0.15-0.7 μg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 15 kDa. The predicted N-terminus is Thr 103. DTT-reduced Protein migrates as 18-23 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Vascular endothelial growth factor C is also known as VEGFC, Flt4-L and VRP; it contains the C-terminal propeptide which has an unusual structure with tandemly repeated cysteine-rich motifs. Upon biosynthesis, VEGFC is secreted as a non-covalent momodimer in an anti-parallel fashion. VEGFC is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. The structure and function of this protein is similar to those of vascular endothelial growth factor D (VEGF-D). VEGFC may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Overexpression of VEGF-C causes lymphatics to enlarge possibly facilitates metastasis.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans