Biovision
Human CellExp™ Cystatin C, Human Recombinant
- SKU:
- 26-4878
- Availability:
- Usually Shipped in 5 Working Days
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Blue ice
- Shelf Life:
- 12 months
Description
Biomolecule/Target: N/A
Synonyms: Human Recombinant Cystatin C
Alternates names: Cystatin-3, Gamma-trace neuroendocrine basic polypeptide, Post-gamma-globulin
Taglines: A biomarker of kidney function
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 1472
NCBI Gene Symbol: CST4
Gene Source: Human
Accession #: P01036
Recombinant: Yes
Source: Human 293 cells
Purity by SDS-PAGE #: ≥95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: <0.1 ng/μg
Activity (Specifications/test method): N/A
Biological activity: The inhibitory function of Cystatin C on papin’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 µg/mL (0.3 to 1.5 µM) with a range of 1.56 µg/mL to 50 µg/mL Cystatin C in presence of 0.55 µM papain and 0.44 µM L-BAPA.
Results: Typically 0.3 to 1.5 µM IC50.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 12-13 kDa
Concentration: N/A
Appearance: Lyophilized protein
Physical form description: Lyophilized in PBS
Reconstitution Instructions: Reconstitute in sterile PBS.
Background Information: Xeno-free Cystatin C HuXp (Cys-C) is expressed in human 293 cells as a monomer with an apparent molecular mass of 12 to 13 kDa. Native Cys-C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting new onset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans