Human CellExp™ CD155, human recombinant

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SKU:
26-7462
Availability:
Usually Shipped in 5 Working Days
Storage Temperature:
-20°C
Shipping Conditions:
Gel Pack
Shelf Life:
12 months
£233.06 - £673.38
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Description

Biomolecule/Target: CD155

Synonyms: PVR, FLJ25946, PVS, CD155, TAGE4, HVED, NECL5, cluster of differentiation 155.

Alternates names: PVR, FLJ25946, PVS, CD155, TAGE4, HVED, NECL5, cluster of differentiation 155.

Taglines: Establishes intercellular adherens junctions between epithelial cells

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 5817

NCBI Gene Symbol: PVR

Gene Source: Human

Accession #: P15151

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to bind with recombinant human DNAM1 / CD226 in a functional ELISA assay

Results: Measured by its ability to bind with recombinant human DNAM1 / CD226 in a functional ELISA assay

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with a C-terminal 6×his tag and has a calculated MW of 28 kDa. The predicted N-terminus is Trp21. DTT-reduced protein migrates as 50-65 kDa polypeptide in SDS-PAGE resulting from glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The full length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains. It has 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy[1] while a high resolution structures of the ectodomain D1 and D2 of CD155 were solved by x-ray crystallography.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans

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