Human CellExp™ CD19, Fc Tag, Human recombinant

Biomolecule/Target: CD19

Synonyms: CD19, B4, CVID3, MGC12802

Alternates names: CD19, B4, CVID3, MGC12802

Taglines: B-lymphocyte antigen CD19

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 930

NCBI Gene Symbol: CD19

Gene Source: Human

Accession #: AAH06338

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: >90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized FMC63 mAb at 2 μg/mL (100 μL/well) can bind Human CD19, Fc Tag with a linear range of 0.15-1.25 μg/mL

Results: N/A

Binding Capacity: Immobilized FMC63 mAb at 2 μg/mL (100 μL/well) can bind Human CD19, Fc Tag with a linear range of 0.15-1.25 μg/mL

Unit Definition: N/A

Molecular Weight: 55.1 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Generally Mannitol or Trehalose is added as a protectant before lyophilization

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 50 μg/ml.

Background Information: B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans