Human CellExp™ CD200/OX-2, human recombinant

Biomolecule/Target: CD200/OX-2

Synonyms: CD200, MOX1, MOX2, MRC, OX-2, My033

Alternates names: CD200, MOX1, MOX2, MRC, OX-2, My033

Taglines: Involved in regulation of macrophage and mast cell function.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4345

NCBI Gene Symbol: CD200

Gene Source: Human

Accession #: P41217

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 μg/ml. The concentration of rhCD200 that produces 50% of the optimal binding response is approximately 0.5‑16 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD200R1 at 2 μg/ml. The concentration of rhCD200 that produces 50% of the optimal binding response is approximately 0.5‑16 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 23.4 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 35-45 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: CD200 also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200 / OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2 / CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans