Human CellExp™ CD73 / NT5E, Human recombinant

Biomolecule/Target: CD73 / NT5E

Synonyms: CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE

Alternates names: CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE

Taglines: Enzyme that convert AMP to adenosine

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4907

NCBI Gene Symbol: NT5E

Gene Source: Human

Accession #: P21589-1

Recombinant: TRUE

Source: HEK293

Purity by SDS-PAGE #: >85% as determined by SDS-PAGE.

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 30 kDa, 40 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. It is recommended to store at -20°C.

Background Information: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Amino acid sequence: AA Trp 27 - Ser 549

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans