Human CellExp™ Decorin /Bone proteoglycan II, human recombinant

Biomolecule/Target: Decorin /Bone proteoglycan II

Synonyms: Decorin, DCN, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B.

Alternates names: Decorin, DCN, CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B.

Taglines: A protein which binds to type I collagen fibrils, and plays a role in matrix assembly

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 1634

NCBI Gene Symbol: DCN

Gene Source: Human

Accession #: P07585

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rh Decorin can significantly delay the rate of type I collagen fibrillogenesis.

Results: Measured by its ability to modulate collagen fibrillogenesis. At 5 µg/mL, rh Decorin can significantly delay the rate of type I collagen fibrillogenesis.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein fused with 6×His tag at the C-terminus, has a calculated MW of 38.8 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 45 kDa or higher due to different glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Decorin, also known as bone proteoglycan II, PGS2, SLRR1B, DCN, DSPG2 and PG40, is a secreted chondroitin /dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin appears to influence fibrillogenesis, and also interacts with fibronectin, thrombospondin, the complement component C1q, epidermal growth factor receptor (EGFR) and transforming growth factor-beta (TGF-beta). Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD).

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans