Human CellExp™ EGF, human recombinant

Biomolecule/Target: EGF

Synonyms: EGF, URG, HOMG4, epidermal growth factor

Alternates names: EGF, URG, HOMG4, epidermal growth factor

Taglines: Plays an important role in the regulation of cell growth

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 1950

NCBI Gene Symbol: EGF

Gene Source: Human

Accession #: P01133

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

Biological activity: ED50 is typically in a single digit ng/mL.

Results: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically in a single digit ng/mL.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 7 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered PBS (pH 7.4) with 5% trehalose.

Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4 up to 0.2 µg/µl.

Background Information: Human epidermal growth factor (EGF) is also known as HOMG4 and URG, and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans