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Human CellExp™ EPOR/Erythropoietin Receptor, human recombinant

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SKU:
26-7454
Availability:
Usually Shipped in 5 Working Days
Storage Temperature:
-20°C
Shipping Conditions:
Gel Pack
Shelf Life:
12 months
€284.00 - €820.00
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Description

Biomolecule/Target: EPOR/Erythropoietin Receptor

Synonyms: EPOR, Erythropoietin Receptor.

Alternates names: EPOR, Erythropoietin Receptor.

Taglines: A major glycoprotein hormone regulator of mammalian erythropoiesis

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2057

NCBI Gene Symbol: EPOR

Gene Source: Human

Accession #: P19235

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is typically 2 - 6 ng/ml in the presence of 0.2 U/ml of rhEpo-Fc.

Results: Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is typically 2 - 6 ng/ml in the presence of 0.2 U/ml of rhEpo-Fc.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 50.1 kDa. The predicted N-terminus is Ala 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR is a type 1 single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR pre-exists as dimers which upon binding of a 34 kDa ligand erythropoietin (EPO), changes its homodimerized state. These conformational changes result in the autophosphorylation of Jak2 kinases that are pre-associated with the receptor. Erythropoietin is necessary to maintain endothelial cells and to promote tumor angiogenesis, hence the dysregulation of EpoR may affect the growth of certain tumors. EpoR signaling prevents neuronal death and ischemic injury.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans

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