Human CellExp™ Erythropoietin Receptor
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: EPOR, EPO-R
Alternates names: EPOR, EPO-R
Taglines: Mediates erythropoietin-induced erythroblast proliferation and differentiation
Country of Animal Origin: USA
NCBI Gene ID #.: 2057
NCBI Gene Symbol: N/A
Gene Source: Human
Accession #: P19235, NP_000112.1
Source: HEK293 cells
Purity by SDS-PAGE #: ≥ 95% by SDS-PAGE
Assay #2: N/A
Endotoxin Level: < 0.1 EU/μg of the protein as determined by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 60-300 ng/ml in the presence of 0.2 U/ml of Recombinant Human EPO Receptor/EPOR.
Results: Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 60-300 ng/ml in the presence of 0.2 U/ml of Recombinant Human EPO Receptor/EPOR.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: The protein migrates as a band at ~55-60 kDa band in SDS-PAGE.
Physical form description: N/A
Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/ml using sterile distilled water
Background Information: Erythropoietin Receptor is a member of the cytokine receptor family. It mediates erythropoietin-induced erythroblast proliferation and differentiation, activation of Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor plays a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.
Amino acid sequence: The target protein is expressed with the sequence (Ala25-Pro250) of human Erythropoietin Receptor protein fused to Fc and 6×His tag at the C-terminus.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans