Human CellExp™ sIL-6 receptor, Human Recombinant

Biomolecule/Target: sIL-6 Receptor α

Synonyms: soluble IL-6 receptor alpha, B cell stimulatory factor-2, CD126, Membrane glycoprotein 80, gp80, IL-6R 1, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA

Alternates names: soluble IL-6 receptor alpha, B cell stimulatory factor-2, CD126, Membrane glycoprotein 80, gp80, IL-6R 1, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA

Taglines: A type I cytokine receptor

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: N/A

NCBI Gene Symbol: IL6R

Gene Source: Human

Accession #: P08887

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥ 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <0.1 EU/μg

Activity (Specifications/test method): N/A

Biological activity: The ED50 was determined by its ability to intensify the IL-6 induced growth inhibition of murine M1 cells is ≤ 5.0 ng/ml, in the presence of 20 ng/ml of rhIL-6.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 37.6 kDa

Concentration: N/A

Appearance: lyophilized powder

Physical form description: Lyophilized from 1x PBS pH 7.2.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Background Information: IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. The IL-6Rα chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6Rα. The gp130 also can transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6Rα which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. Recombinant human sIL-6Rα is a 37.6 kDa protein consisting of the extracellular domain of the IL-6Rα chain (338 amino acid residues).

Amino acid sequence: LAPRRCPAQ EVARGVLTS LPGDSVTLT CPGVEPEDN ATVHWVLRK PAAGSHPSR WAGMGRRLL LRSVQLHDS GNYSCYRAG RPAGTVHLL VDVPPEEPQ LSCFRKSPL SNVVCEWGP RSTPSLTTK AVLLVRKFQ NSPAEDFQE PCQYSQESQ KFSCQLAVP EGDSSFYIV SMCVASSVG SKFSKTQTF QGCGILQPD PPANITVTA VARNPRWLS VTWQDPHSW NSSFYRLRF ELRYRAERS KTFTTWMVK DLQHHCVIH DAWSGLRHV VQLRAQEEF GQGEWSEWS PEAMGTPWT ESRSPPAEN EVSTPMQAL TTNKDDDNI LFRDSANAT SLPVQD

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans