Human CellExp™ FABP2 /I-FABP, human recombinant

Biomolecule/Target: FABP2 /I-FABP

Synonyms: FABP2, FABPI, I-FABP

Alternates names: FABP2, FABPI, I-FABP

Taglines: Involved in triglyceride-rich lipoprotein synthesis

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2169

NCBI Gene Symbol: FABP2

Gene Source: Human

Accession #: P12104

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: The binding affinity of Recombinant Human FABP2/I‑FABP for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half-maximal fluorescence of 3 µM Recombinant Human FABP2/I‑FABP is achieved with approximately 3 µM cis‑paranaric acid.

Results: The binding affinity of Recombinant Human FABP2/I‑FABP for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half-maximal fluorescence of 3 µM Recombinant Human FABP2/I‑FABP is achieved with approximately 3 µM cis‑paranaric acid.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 16 kDa. The predicted N-terminus is Ala 2. DTT-reduced Protein migrates as 16 kDa.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Fatty acid-binding protein 2 (FABP2), is also known as Fatty acid-binding protein, intestinal (FABPI), Intestinal-type fatty acid-binding protein (I-FABP). FABP2 belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. FABP2 / FABPI is expressed in the small intestine and at much lower levels in the large intestine and is highest expressed in the jejunum. FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. FABP2 binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans