Human CellExp™ FGF-2/FGF-basic, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant FGF-basic

Alternates names: Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Taglines: A growth factor involved in angiogenesis, wound healing and embryonic development

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2247

NCBI Gene Symbol: FGF2

Gene Source: Human

Accession #: P09038

Recombinant: Yes

Source: Human cell expressed

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line.

Results: 0.1 to 0.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17 kDa, monomer, non-glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from a PBS solution.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

Background Information: FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans