Human CellExp™ Furin, human recombinant
- Usually Shipped in 5 Working Days
- 10 μg
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: FURIN, FUR, PACE, PCSK3, SPC1
Alternates names: FURIN, FUR, PACE, PCSK3, SPC1
Taglines: Involved in processing of latent precursor proteins into their biologically active products
Country of Animal Origin: USA
NCBI Gene ID #.: 5045
NCBI Gene Symbol: PACE
Gene Source: Human
Accession #: P09958
Source: HEK 293 cells
Purity by SDS-PAGE #: ≥95%
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method): Measured by its ability to cleave the fluorogenic peptide substrate p – Glu – Arg – Thr – Lys – Arg – AMC. The bioactivity was measured in 100 μL reaction mixture containing 4 µg/ml of rhFurin, 50 μM substrate, 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij35, pH 9.0. The specific activity is >130 pmol/min/µg.
Biological activity: Measured by its ability to cleave the fluorogenic peptide substrate p – Glu – Arg – Thr – Lys – Arg – AMC. The bioactivity was measured in 100 μL reaction mixture containing 4 µg/ml of rhFurin, 50 μM substrate, 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij35, pH 9.0. The specific activity is >130 pmol/min/µg.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 66.5 kDa. The predicted N-terminus is Asp 108. DTT-reduced Protein migrates as 55-66 kDa.
Appearance: Lyophilized powder
Physical form description: Lyophilized from 0.22 μm filtered solution in MES, Brij-35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Furin also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg'). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans