Human CellExp™ Furin, human recombinant

Biomolecule/Target: Furin

Synonyms: FURIN, FUR, PACE, PCSK3, SPC1

Alternates names: FURIN, FUR, PACE, PCSK3, SPC1

Taglines: Involved in processing of latent precursor proteins into their biologically active products

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 5045

NCBI Gene Symbol: PACE

Gene Source: Human

Accession #: P09958

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): Measured by its ability to cleave the fluorogenic peptide substrate p – Glu – Arg – Thr – Lys – Arg – AMC. The bioactivity was measured in 100 μL reaction mixture containing 4 µg/ml of rhFurin, 50 μM substrate, 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij­35, pH 9.0. The specific activity is >130 pmol/min/µg.

Biological activity: Measured by its ability to cleave the fluorogenic peptide substrate p – Glu – Arg – Thr – Lys – Arg – AMC. The bioactivity was measured in 100 μL reaction mixture containing 4 µg/ml of rhFurin, 50 μM substrate, 25 mM Tris, 1 mM CaCl2, 0.5% (w/v) Brij­35, pH 9.0. The specific activity is >130 pmol/min/µg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 66.5 kDa. The predicted N-terminus is Asp 108. DTT-reduced Protein migrates as 55-66 kDa.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 μm filtered solution in MES, Brij-35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Furin also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg'). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans