Human CellExp™ GITR / TNFRSF18, Human recombinant

Biomolecule/Target: GITR

Synonyms: AITR, GITR, TNFRSF18, CD357

Alternates names: AITR, GITR, TNFRSF18, CD357

Taglines: Tumor necrosis factor receptor superfamily member 18

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 8784

NCBI Gene Symbol: TNFRSF18

Gene Source: Human

Accession #: Q9Y5U5-1

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: ≥92%

Assay #2: N/A

Endotoxin Level: < 1 EU/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Human GITR/TNFRSF18, His Tag at 2 µg/mL (100 µL/well) can bind r-Human GITR Ligand /TNFSF18 with a linear range of 0.8-25 ng/mL.

Results: N/A

Binding Capacity: Immobilized Human GITR/TNFRSF18, His Tag at 2 µg/mL (100 µL/well) can bind r-Human GITR Ligand /TNFSF18 with a linear range of 0.8-25 ng/mL.

Unit Definition: N/A

Molecular Weight: This protein is fused with a 6× His tag at C-terminus and has a calculated MW of 15.4 kDa. The protein migrates as 22-25 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Background Information: Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans