Human CellExp™ Glypican 2 / GPC2, Mouse Recombinant

Biomolecule/Target: N/A

Synonyms: Glypican 2, GPC2

Alternates names: Glypican 2, GPC2

Taglines: Cell surface proteoglycan that bears heparan sulfate.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 71951

NCBI Gene Symbol: Gpc2

Gene Source: Mouse

Accession #: Q8BKV1

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Recombinant Human Midkine Protein, CF at 2 μg/mL (100 μL/well) can bind Mouse Glypican 2, His Tag with a linear range of 2-13 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.6 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE).

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Amino acid sequence: AA His 22 - Ser 556

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans