Human CellExp™ TNF-alpha Extracellular Domain, Human Recombinant

Biomolecule/Target: N/A

Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2

Alternates names: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2

Taglines: A potent cytokine exerting cytotoxic effects on a wide range of cells

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 7124

NCBI Gene Symbol: TNFA

Gene Source: Human

Accession #: P01375

Recombinant: TRUE

Source: HEK 293 cells

Purity by SDS-PAGE #: > 95% by SDS - PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): TNF-alpha’s cytotoxicity effect was measured in a cell based assay with BioVision’s Colorimetric Assay kit K302. The ED50 for this effect is typically less than 1ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18 kDa with a 6×His tag at the C-terminus

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Background Information: Tumor Necrosis Factor-α (TNF-α) is a highly pleiotropic cytokine with multiple activities other than its originally discovered role of tumor necrosis in rodents. It contributes to both inflammations during infections and anti-inflammatory/tissue repair processes after clearance of infection. TNF-α is mainly produced by activated macrophages, T lymphocytes, and natural killer (NK) cells. The activity of TNF-α is mediated through two cell surface receptors, TNF-R1 (p55) and TNF-R2 (p75) that differ in their signaling activity. TNF-α's primary role is in the regulation of immune cells. It can induce cell death of certain tumor cell lines. About 28% of cancers are susceptible to direct cell killing mediated by soluble TNF-α.

Amino acid sequence: Val 77 – Thr 233

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans