Human CellExp™ Human CD40/ TNFRSF5, Human recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel pack
- Shelf Life:
- 12 months
Synonyms: CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50
Alternates names: CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50
Taglines: Tumor necrosis factor receptor superfamily member 5
Country of Animal Origin: USA
NCBI Gene ID #.: 958
NCBI Gene Symbol: CD40
Gene Source: Human
Accession #: AAH12419.1
Source: HEK 293 cells
Purity by SDS-PAGE #: >95%
Assay #2: N/A
Endotoxin Level: < 1 EU/μg
Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.
Biological activity: Immobilized Human CD40, His Tag at 1 µg/mL (100 µL/well) can bind Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.
Binding Capacity: Immobilized Human CD40, His Tag at 1 µg/mL (100 µL/well) can bind Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.
Unit Definition: N/A
Molecular Weight: This protein is fused with a 6× His tag at C-terminus and has a calculated MW of 20 kDa. The protein migrates as 32 kDa in SDS-PAGE due to glycosylation.
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml
Background Information: Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans