Human CellExp™ Human CD40/ TNFRSF5, Human recombinant

Biomolecule/Target: CD40

Synonyms: CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50

Alternates names: CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50

Taglines: Tumor necrosis factor receptor superfamily member 5

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 958

NCBI Gene Symbol: CD40

Gene Source: Human

Accession #: AAH12419.1

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: SDS-PAGE

Purity: >95%

Assay #2: N/A

Endotoxin Level: < 1 EU/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Human CD40, His Tag at 1 µg/mL (100 µL/well) can bind Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.

Results: N/A

Binding Capacity: Immobilized Human CD40, His Tag at 1 µg/mL (100 µL/well) can bind Human CD40 Ligand /TNFSF5 with a linear range of 0.01-0.12 ng/mL.

Unit Definition: N/A

Molecular Weight: This protein is fused with a 6× His tag at C-terminus and has a calculated MW of 20 kDa. The protein migrates as 32 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Background Information: Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans