Human CellExp™ ICOS / CD278, Mouse Recombinant

Biomolecule/Target: N/A

Synonyms: ICOS, CD278, AILIM, Inducible T-cell costimulator

Alternates names: ICOS, CD278, AILIM, Inducible T-cell costimulator

Taglines: Inducible T-cell costimulator

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 54167

NCBI Gene Symbol: Icos

Gene Source: Mouse

Accession #: Q9WVS0-1

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: >90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): N/A

Biological activity: Immobilized Human B7-H2 at 1 μg/mL (100 μL/well) can bind Mouse ICOS with a linear range of 0.01-0.2ng/mL

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.7 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

Amino acid sequence: Glu 21 - Leu 144

Handling: Centrifuge the vial prior to opening.

Usage: N/A