Human CellExp™ TPO, Mouse Recombinant

Biomolecule/Target: N/A

Synonyms: Thpo, C-mpl ligand,ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1

Alternates names: Thpo, C-mpl ligand,ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand, Mgdf, Ml, Mpllg, Tpo, thrombopoietin isoform 1

Taglines: Stimulates the proliferation and maturation of megakaryocytes

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 21832

NCBI Gene Symbol: Thpo

Gene Source: Mouse

Accession #: P40226

Recombinant: TRUE

Source: HEK 293 cells

Purity by SDS-PAGE #: >90%

Assay: SDS - PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Activity (Specifications/test method): Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤4ng/ml.

Biological activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤4ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 36.4 kDa (His-tag at C-terminus)

Concentration: N/A

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions: N/A

Background Information: Thrombopoietin (THPO), also known as megakaryocyte growth and development factor (MGDF), is a glycoprotein hormone produced by the liver and kidney which regulates the production of platelets. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Megakaryocytopoiesis is the cellular development process that leads to platelet production. It is a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Recombinant mouse Thrombopoietin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Amino acid sequence: aa 22-356

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans