Human CellExp™ IL-12, Mouse Recombinant

Biomolecule/Target: N/A

Synonyms: NKSF, CLMF

Alternates names: NKSF, CLMF, Cytotoxic lymphocyte maturation factor 40 kDa subunit

Taglines: A T cell-stimulating factor

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 16160

NCBI Gene Symbol: IL12

Gene Source: Mouse

Accession #: P43432

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: ≥95%

Assay #2: HPLC

Endotoxin Level: <0.1 EU/μg purified protein

Activity (Specifications/test method): N/A

Biological activity: The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL.

Results: 0.1 ng/mL

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 70 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Recombinant mouse IL-12 is lyophilized from 0.5 x PBS, pH 7.5.

Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Background Information: Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 subunit (35 kDa) and one p40 subunit (40 kDa), with a total of 506 amino acids and a molecular weight of approximately 70 kDa.

Amino acid sequence: p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans