Human CellExp™ IL-4 Rα, Human Recombinant

Biomolecule/Target: IL-4 Rα

Synonyms: soluble IL-4 receptor alpha, CD124

Alternates names: soluble IL-4 receptor alpha, CD124

Taglines: A receptor for both interleukin 4 and interleukin 13

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3566

NCBI Gene Symbol: IL4RA

Gene Source: Human

Accession #: P24394

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.

Biological activity: Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~24 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS pH 7.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to - 80°C

Background Information: IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain (γc). The type I receptor contains in addition to the γc an IL-4Rα subunit, whereas the type II receptor contains the IL-13Rα. The secreted extracellular domain of IL-4Rα, called sIL-4Rα, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naïve CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4Rα is a 209 amino acid protein which corresponds to the entire extracellular domain of IL- 4Rα.

Amino acid sequence: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans