Human CellExp™ IL-4, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant IL-4

Alternates names: BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.

Taglines: A key regulator in humoral and adaptive immunity

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3565

NCBI Gene Symbol: IL4

Gene Source: Human

Accession #: P05112

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically 0.1 to 0.5 ng/mL. The specific activity was determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Results: 0.1 to 0.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 14 and 19 kDa, monomer

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from a solution of 20 mM Tris-HCl, pH 7.4, with 300 mM NaCl.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Background Information: IL4 is a pleiotropic cytokine and a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. It is produced by activated T cells and is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans