Human CellExp™ LIF, human recombinant

Biomolecule/Target: LIF

Synonyms: LIF, CDF, DIA, HILDA, MLPLI

Alternates names: LIF, CDF, DIA, HILDA, MLPLI

Taglines: Affects cell growth and development

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3976

NCBI Gene Symbol: LIF

Gene Source: Human

Accession #: P15018

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg.

Biological activity: Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 <0.1 ng/ml, corresponding to a specific activity of >1X10^7 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: Calculated MW of 19.9 kDa with Gly-Pro at the N- terminus. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 35-45 kDa.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans